• 特异性Please note: Low dilutions of this antibody can cause high background. Please use as high a dilution as possible. Optimal working dilutions are batch dependent.
• 经测试应用WB, ICC, ICC/IF, IHC-FrFl, IHC-FoFr, IHC-Fr, IHC-Pmore details
• 种属反应性
  与反应: Mouse, Rat, Chicken, Cat, Human, Cynomolgus Monkey, Quail, Rhesus monkey
• 免疫原

  Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Doublecortin.

  参阅Abcam的专有抗源政策

   

  (Peptide available as ab19804.)

• 阳性对照
  • This antibody gave a positive signal in Brain (Mouse) Tissue Lysate - normal tissue, 0 days old

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• 浓度Batch dependent within range: 50 µg 浓度为 0.9 - 1 mg/ml 
  

  查找您批次的浓度
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Cell Adhesion Proteins
   
  • Cytoskeletal Proteins
   
  • Microtubules

  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Soma marker

  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Growth Cone

  • Tags & Cell Markers
   
  • Cell Type Markers
   
  • Neuroscience Markers
   
  • Neuronal

应用

靶标

• 功能Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
• 组织特异性Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
• 疾病相关Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
  Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
  Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
• 序列相似性Contains 2 doublecortin domains.
• 细胞定位Cytoplasm.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 1641 Human
  • Entrez Gene: 13193 Mouse
  • Entrez Gene: 84394 Rat
  • Omim: 300121 Human
  • SwissProt: O43602 Human
  • SwissProt: O88809 Mouse
  • SwissProt: Q9ESI7 Rat
  • Unigene: 34780 Human

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