TRAIL小鼠ELISA试剂盒-试剂盒-抗体-生物在线

产品名称： TRAIL小鼠ELISA试剂盒

英文名称： Apo 2 ligand APO2L CD253 Protein TRAIL TL2 TNF related apoptosis inducing ligand Tumor necrosis factor ligand superfamily member 10

产品编号： ab193730

产品价格： null

产品产地：英国

品牌商标： abcam

更新时间： null

使用范围：

深圳市宇德立生物科技有限公司

进入展台

精确度

批次内
样品	n	Mean	SD	CV%
overall				< 10%

复检

实验步骤One step assay
种属反应性
与反应: Mouse
产品概述

Abcam’s TRAIL (TNF-related apoptosis-inducing ligand) Mouse ELISA Kit (ab193730) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TRAIL in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse TRAIL coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TRAIL present in the samples. The wells are washed and biotinylated anti-mouse TRAIL antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TRAIL bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
经测试应用Sandwich ELISAmore details
平台Pre-coated microplate (12 x 8 well strips)

组件	1 x 96 tests
20X Wash Buffer Concentrate	1 x 25ml
250X HRP-Streptavidin Concentrate	1 x 200µl
5X Assay Diluent B	1 x 15ml
Assay Diluent A	1 x 30ml
Biotinylated mouse TRAIL detection antibody	2 vials
mouse TRAIL standards	2 vials
Pre-coated mouse TRAIL Microplate	1 unit
Stop Solution :sulfuric acid.	1 x 8ml
TMB One-Step Substrate Reagent	1 x 12ml

相关性TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
细胞定位Cell Membrane; single-pass type II membrane protein.
别名
- Apo 2 ligand
- APO2L
- CD253
- Protein TRAIL
- TL2
- TNF related apoptosis inducing ligand
- Tumor necrosis factor ligand superfamily member 10
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