人次黄嘌呤磷酸核糖基转移酶1(HPRT1)多克隆抗体
Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
[PROPERTIES]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; IHC; ICC; IP
[ IMMUNOGEN]
Immunogen:Recombinant HPRT1 (Thr3~Ala218) expressed in E.coli
[ APPLICATIONS]
Western blotting: 0.5-5µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
[FORMULATION]
Form & Buffer:Supplied as solution form in 0.01M PBS, pH7.4, containing0.05% Proclin-300, 50% glycerol.
[ QUALITY CONTROL ] 
Content:The quality control contains recombinant HPRT1 disposed in loadingbuffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, 0.02%NaN₃ 
[STORAGE AND STABILITY] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[IDENTIFICATION]

Figure 1. Western Blot
A. Sample: Recombinant HPRT1, Human B. Lane1: Rat Liver Tissue Lane2: Rat Brain Tissue Lane3: Human Hela Cells Lane4: Human HepG2 Cells Lane5: Human A549 Cells Primary Ab: 3µg/mL Rabbit Anti-Human HPRT1 Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig

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