山羊Anti-Rabbit IgG H&L (HRP)-抗体-抗体-生物在线
山羊Anti-Rabbit IgG H&L (HRP)

山羊Anti-Rabbit IgG H&L (HRP)

商家询价

产品名称： 山羊Anti-Rabbit IgG H&L (HRP)

英文名称： 山羊Anti-Rabbit IgG H&L (HRP)

产品编号： ab97051

产品价格： null

产品产地： 英国

品牌商标： abcam

更新时间： null

使用范围：

深圳市宇德立生物科技有限公司
  • 联系人 :
  • 地址 : 深圳市宝安区西乡宝民二路贤基大厦4E
  • 邮编 :
  • 所在区域 : 广东
  • 电话 : 133****4454
  • 传真 : 0755-26457513
  • 邮箱 : 1484332550@qq.com
进入展台

 

  • 靶标种属Rabbit
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用ICC, IHC-P, ELISA, WBmore details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • 浓度1 mg 浓度为 1 mg/ml 
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明Molar enzyme/ antibody protein ratio is 4:1
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Part of the AbExcel range.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Abreviews 说明
ICC   Use at an assay dependent dilution.
IHC-P   1/200 - 1/5000.
ELISA   1/10000 - 1/100000. (Primary).
WB   1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Goat Anti-Rabbit IgG H&L (HRP) 图像

  • Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)This image is courtesy of an anonymous Abreview.

    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97051, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051)
    Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds