重组T7 RNA聚合酶
采用大肠杆菌表达来源于T7噬菌体的RNA聚合酶基因,该酶具有高度的专一性,在镁离子存在的情况下,只会以含有T7启动子序列的单链或双链DNA为模板( 5’-TAATACGACT CACTATAG*-3’),以NTP为底物
ThermoFisher| 11789-100| Gateway™ BP Clonase™ II Enzyme mix
ThermoFisher 11789-100 Gateway™ BP Clonase™ II Enzyme mix
ThermoFisher| Q33216 |Qubit™ 3 Fluorometer
ThermoFisher| Q33216ThermoFisher| Q33216ThermoFisher| Q33216ThermoFisher| Q33216
ThermoFisher| S-012-5| EpiLife™ Defined Growth Supplement (EDGS)
ThermoFisher S-012-5 EpiLife™ Defined Growth Supplement (EDGS)
ThermoFisher|88291| Pierce™ D-Luciferin, Monosodium Salt
ThermoFisher 88291 Pierce™ D-Luciferin, Monosodium Salt
INVITROGEN| PA1-27956| TGFBR2 Polyclonal Antibody
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ThermoFisher|MA511544| Anti-Actin Smooth Muscle
ThermoFisher MA511544 Anti-Actin Smooth Muscle
INVITROGEN |11789013 |Gateway™ BP Clonase™ Enzyme Mix
INVITROGEN 11789013 Gateway™ BP Clonase™ Enzyme Mix
ThermoFisher |10099141| FBS QUALIFIED AUSTRALIA ORIGIN
ThermoFisher |10099141|ThermoFisher |10099141|ThermoFisher |10099141|ThermoFisher |10099141|